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December 26, 2018
DOI:
1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center
Summary
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Aquí, presentamos cadena montaje gRNA clonación (fantasía STAgR), un método para multiplexar fácilmente gRNA vectores para CRISPR/Cas9 enfoques. Fantasía STAgR hace gRNA multiplexación simple, eficiente y adaptable.
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Breunig, C. T., Neuner, A. M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S. H. A Customizable Protocol for String Assembly gRNA Cloning (STAgR). J. Vis. Exp. (142), e58556, doi:10.3791/58556 (2018).
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