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Eine anpassbare Protokoll für String Montage gRNA Klonen (STAgR)
JoVE Journal
Genetics
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JoVE Journal Genetics
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
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Eine anpassbare Protokoll für String Montage gRNA Klonen (STAgR)

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9,350 Views

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10:00 min

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December 26, 2018

DOI:

10.3791/58556-v

DOI:
10.3791/58556-v

•

10:00 min
December 26, 2018

•

4 Views
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1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center

Summary

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Hier präsentieren wir Zeichenfolge Montage gRNA Klonen (STAgR), eine Methode, um leicht gRNA Vektoren multiplex für nähert sich CRISPR/Cas9. STAgR macht gRNA Multiplexen, einfach, effizient und individuell.

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Cite this Article

Breunig, C. T., Neuner, A. M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S. H. A Customizable Protocol for String Assembly gRNA Cloning (STAgR). J. Vis. Exp. (142), e58556, doi:10.3791/58556 (2018).

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