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Un protocole personnalisable pour chaîne Assemblée gRNA clonage (STAgR)
JoVE Journal
Genetics
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JoVE Journal Genetics
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
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Un protocole personnalisable pour chaîne Assemblée gRNA clonage (STAgR)

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9,350 Views

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10:00 min

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December 26, 2018

DOI:

10.3791/58556-v

DOI:
10.3791/58556-v

•

10:00 min
December 26, 2018

•

4 Views
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1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center

Summary

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Nous présentons ici la chaîne Assemblée gRNA clonage (STAgR), une méthode pour multiplexer facilement gRNA vecteurs pour les approches de CRISPR/Cas9. STAgR rend gRNA multiplexage simple, efficace et personnalisable.

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Cite this Article

Breunig, C. T., Neuner, A. M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S. H. A Customizable Protocol for String Assembly gRNA Cloning (STAgR). J. Vis. Exp. (142), e58556, doi:10.3791/58556 (2018).

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