Journal
/
Genetics
/
Um protocolo personalizáveis para String Assembly gRNA clonagem (STAgR)
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
A Customizable Protocol for String Assembly gRNA Cloning (STAgR)

Um protocolo personalizáveis para String Assembly gRNA clonagem (STAgR)

português

Automatically Generated

9,350 Views

•

10:00 min

•

December 26, 2018

DOI:

10.3791/58556-v

DOI:
10.3791/58556-v

•

10:00 min
December 26, 2018

•

4 Views
, , , , ,
1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center

Summary

Automatically generated

Aqui, apresentamos a sequência montagem gRNA clonagem (STAgR), um método facilmente multiplexar gRNA vetores para CRISPR/Cas9 aproxima-se. STAgR faz a multiplexação de gRNA simples, eficiente e personalizável.

Explore More Videos

CRISPR-Cas9GRNA CloningSTAgRMultiplex GRNAGibson AssemblyDNA TemplatePCRMagnetic BeadsDNA Purification

Read Article

Cite this Article

Breunig, C. T., Neuner, A. M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S. H. A Customizable Protocol for String Assembly gRNA Cloning (STAgR). J. Vis. Exp. (142), e58556, doi:10.3791/58556 (2018).

Download .ris file

Copy

Share Video

.

Copy