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December 26, 2018
DOI:
1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center
Summary
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Qui, presentiamo la stringa Assemblea gRNA clonazione (STAgR), un metodo per multiplex facilmente gRNA vettori per CRISPR/Cas9 si avvicina. STAgR rende gRNA multiplexing semplice, efficiente e personalizzabile.
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Breunig, C. T., Neuner, A. M., Giehrl-Schwab, J., Wurst, W., Götz, M., Stricker, S. H. A Customizable Protocol for String Assembly gRNA Cloning (STAgR). J. Vis. Exp. (142), e58556, doi:10.3791/58556 (2018).
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