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生理活性とエネルギー依存性タンパク質輸送アッセイでの使用の分離

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

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生理活性とエネルギー依存性タンパク質輸送アッセイでの使用の分離

10,060 Views

•

12:25 min

•

September 28, 2018

DOI:

10.3791/58393-v

DOI:

10.3791/58393-v

•

12:25 min

September 28, 2018

•

1 Views

Anthony Asher¹, Iniyan Ganesan¹, Laura Klasek¹, Steven M. Theg¹

¹Department of Plant Biology,University of California – Davis

Summary

生理活性チラコイドの高収率分離のためにここのプロトコルおよび葉緑体ツインアルギニン転流 (cpTat)、分泌 (cpSec1)、およびシグナル認識粒子 (cpSRP) のための蛋白質輸送アッセイを提案します。

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