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生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
DOI:

12:25 min

September 28, 2018

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Chapters

  • 00:04Title
  • 00:30Chloroplast Isolation and Quantification
  • 03:30Isolation of Thylakoids
  • 04:42Stromal Extract (SE) Recovery
  • 06:33Transport through the cpTat Pathway
  • 07:44Transport through the cpSec1 and cpSRP Pathways
  • 08:59Thermolysin Treatment
  • 10:16Results: Transport of Proteins Through cpTat and cpSec1 and Integration Through cpSRP Pathways
  • 11:18Conclusion

Summary

Automatic Translation

本文提出了用于叶绿体双精氨酸易位 (cpTat)、分泌 (cpSec1) 和信号识别粒子 (cpSRP) 通路的生理活性类囊体和蛋白质转运测定的高产量隔离的协议。

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