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Isolierung des physiologisch aktiven Thylakoids und deren Einsatz in Energie-abhängige Protein Transport Assays
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
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Isolierung des physiologisch aktiven Thylakoids und deren Einsatz in Energie-abhängige Protein Transport Assays

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10,060 Views

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12:25 min

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September 28, 2018

DOI:

10.3791/58393-v

DOI:
10.3791/58393-v

•

12:25 min
September 28, 2018

•

1 Views
, , ,
1Department of Plant Biology,University of California – Davis

Summary

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Wir präsentieren Protokolle hierin für High Yield Isolierung des physiologisch aktiven Thylakoids und Protein Transport Assays für die Chloroplasten Twin Arginin Translokation (CpTat), sekretorische (cpSec1) und Signalwege Anerkennung Teilchen (CpSRP).

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Cite this Article

Asher, A., Ganesan, I., Klasek, L., Theg, S. M. Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays. J. Vis. Exp. (139), e58393, doi:10.3791/58393 (2018).

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