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Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

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12:25 min

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September 28, 2018

DOI:

10.3791/58393-v

DOI:
10.3791/58393-v

•

12:25 min
September 28, 2018

•

1 Views
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1Department of Plant Biology,University of California – Davis

Summary

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We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.

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Cite this Article

Asher, A., Ganesan, I., Klasek, L., Theg, S. M. Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays. J. Vis. Exp. (139), e58393, doi:10.3791/58393 (2018).

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