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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
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Biochemistry
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JoVE Journal Biochemistry
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

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08:53 min

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October 02, 2017

DOI:

10.3791/56294-v

DOI:
10.3791/56294-v

•

08:53 min
October 02, 2017

•

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1Diamond Light Source,Harwell Science & Innovation Campus, 2Research Complex at Harwell,Harwell Science & Innovation Campus

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Carbohydrate TransportSubstrate-binding ProteinStreptococcus PneumoniaeBiochemical CharacterizationStructural CharacterizationBuffer StabilityOligomerizationCrystallizationSize-exclusion ChromatographyMulti-angle Light ScatteringPre-crystallization TestingProtein ConcentrationCrystallization Screening

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Culurgioni, S., Tang, M., Hall, D. R., Walsh, M. A. Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092. J. Vis. Exp. (128), e56294, doi:10.3791/56294 (2017).

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