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开放补丁夹具移液器测量未搅拌边界层中的离子浓度: 流体流动对离子通道控制的启示
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
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开放补丁夹具移液器测量未搅拌边界层中的离子浓度: 流体流动对离子通道控制的启示

DOI:
10.3791/58228-v

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05:42 min

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January 07, 2019

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, , , , ,
1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science,Konkuk University School of Medicine, 2Department of Emergency Medical Services,Eulji University, 3Department of Mechanical Engineering,Sungkyunkwan University

Chapters

  • 00:04Title
  • 00:30Agarose Salt Bridges Between the Bath Solution and Reference Electrode
  • 01:29Application of Fluid Flow Shear Force to Cells in a Patch-Clamping Chamber
  • 02:21Measuring Changes in Liquid-Metal Junction Potential
  • 03:18Experimental Estimation of Real Chloride Concentration
  • 04:07Results: Effects of Fluid Flow on L-type VDCCL and Kir2.1 Currents With and Without Agarose 3 M KCl Bridge
  • 05:14Conclusion

Summary

Automatic Translation

Automatic Translation

利用膜片钳记录, 从流体流动剪切力敏感性的角度研究了机械敏感离子通道。然而, 根据实验方案, 离子通道流体流动规律的结果可能是错误的。在这里, 我们提供了防止和纠正此类错误的方法, 并提供了理论依据。

Tags

Ion ConcentrationUnstirred Boundary LayerOpen Patch-clamp PipetteFluid FlowIon ChannelsAgarosePotassium ChlorideJunction PotentialVoltage ClampCurrent ClampBathing Solution

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