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Medição da concentração de íons na camada limite Unstirred com pipeta aberto Patch-Clamp: implicações no controle de canais iônicos por fluido fluem
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Biochemistry
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JoVE Journal Biochemistry
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Medição da concentração de íons na camada limite Unstirred com pipeta aberto Patch-Clamp: implicações no controle de canais iônicos por fluido fluem

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05:42 min

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January 07, 2019

DOI:

10.3791/58228-v

DOI:
10.3791/58228-v

•

05:42 min
January 07, 2019

•

2 Views
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1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science,Konkuk University School of Medicine, 2Department of Emergency Medical Services,Eulji University, 3Department of Mechanical Engineering,Sungkyunkwan University

Summary

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Canais de íon Mechanosensitive muitas vezes são estudados em termos de sensibilidade de fluxo fluido/cisalhamento força com gravação de remendo-braçadeira. No entanto, dependendo do protocolo experimental, os resultados no fluxo fluidos-regulamentos de canais iônicos pode ser errôneo. Aqui, nós fornecemos métodos de prevenção e corrigir esses erros com uma base teórica.

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Cite this Article

Kim, J. G., Park, S. W., Shin, K. C., Kim, B., Byun, D., Bae, Y. M. Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow. J. Vis. Exp. (143), e58228, doi:10.3791/58228 (2019).

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