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Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
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Biochemistry
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JoVE Journal Biochemistry
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

English

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6,201 Views

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05:42 min

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January 07, 2019

DOI:

10.3791/58228-v

DOI:
10.3791/58228-v

•

05:42 min
January 07, 2019

•

2 Views
, , , , ,
1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science,Konkuk University School of Medicine, 2Department of Emergency Medical Services,Eulji University, 3Department of Mechanical Engineering,Sungkyunkwan University

Summary

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Mechanosensitive ion channels are often studied in terms of fluid flow/shear force sensitivity with patch-clamp recording. However, depending on the experimental protocol, the outcome on fluid flow-regulations of ion channels can be erroneous. Here, we provide methods for preventing and correcting such errors with a theoretical basis.

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Kim, J. G., Park, S. W., Shin, K. C., Kim, B., Byun, D., Bae, Y. M. Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow. J. Vis. Exp. (143), e58228, doi:10.3791/58228 (2019).

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