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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

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16,895 Views

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11:09 min

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January 05, 2017

DOI:

10.3791/54861-v

DOI:
10.3791/54861-v

•

11:09 min
January 05, 2017

•

5 Views
, ,
1Structural Biology Group,European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences,Keele University, 4Groupe de Microscopie Electronique et Méthodes,Institut de Biologie Structurale

Summary

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The determination of the solution structure of a protein by small angle X-ray scattering (SAXS) requires monodisperse samples. Here, we present two possibilities to ensure minimal delays between sample preparation and data acquisition: online size-exclusion chromatography (SEC) and online ion-exchange chromatography (IEC).

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Brennich, M. E., Round, A. R., Hutin, S. Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline. J. Vis. Exp. (119), e54861, doi:10.3791/54861 (2017).

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