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Biochemistry

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JoVE Journal Biochemistry
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette
 

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Article DOI: 10.3791/58228-v • 05:42 min • January 7th, 2019
January 7th, 2019 •
*1, *2, 1, 1, 3, 1
1Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, 2Department of Emergency Medical Services, Eulji University, 3Department of Mechanical Engineering, Sungkyunkwan University
* These authors contributed equally

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résumé
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Mechanosensitive ion channels are often studied in terms of fluid flow/shear force sensitivity with patch-clamp recording. However, depending on the experimental protocol, the outcome on fluid flow-regulations of ion channels can be erroneous. Here, we provide methods for preventing and correcting such errors with a theoretical basis.

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Keywords: Ion Concentration Unstirred Boundary Layer Open Patch-clamp Pipette Fluid Flow Ion Channels Agarose Potassium Chloride Junction Potential Voltage Clamp Current Clamp Bathing Solution
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