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Weefsel-specifieke miRNA expressie profilering in muis hart secties gebruiken In Situ hybridisatie
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Genetics
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JoVE Journal Genetics
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
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Weefsel-specifieke miRNA expressie profilering in muis hart secties gebruiken In Situ hybridisatie

Nederlands

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8,000 Views

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08:22 min

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September 15, 2018

DOI:

10.3791/57920-v

DOI:
10.3791/57920-v

•

08:22 min
September 15, 2018

•

3 Views
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1Department of Cell and Developmental Biology,University College London, 2Yale Cardiovascular Research Group, Section of Cardiovascular Medicine,Yale School of Medicine, 3Yale Cardiovascular Research Center, Section of Cardiovascular Medicine,Yale School of Medicine

Summary

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Micro-RNAs (miRNAs) zijn korte en zeer homologe RNA-sequenties, post-transcriptional regulatoren van messenger RNAs (mRNAs) bijeenkomen. Huidige miRNA detectiemethoden verschillen in gevoeligheid en specificiteit. We beschrijven een protocol dat in situ hybridisatie en immunokleuring voor gelijktijdige detectie van miRNA en eiwit moleculen op muis hart weefselsecties combineert.

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Cite this Article

Memi, F., Tirziu, D., Papangeli, I. Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization. J. Vis. Exp. (139), e57920, doi:10.3791/57920 (2018).

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