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Hybridation In Situ de Fluorescence de la molécule unique (smFISH) analyse en bourgeonnement levure croissance végétative et la méiose
JoVE Journal
Genetics
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JoVE Journal Genetics
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
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Hybridation In Situ de Fluorescence de la molécule unique (smFISH) analyse en bourgeonnement levure croissance végétative et la méiose

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18,732 Views

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09:28 min

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May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

•

09:28 min
May 25, 2018

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7 Views
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1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

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Cette seule molécule fluorescence in situ hybridation protocole est optimisé afin de quantifier le nombre de molécules d’ARN dans la levure de bière au cours de la méiose et de la croissance végétative.

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Cite this Article

Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

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