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Één molecuul fluorescentie In Situ hybridisatie (smFISH) analyse in Budding vegetatieve groei van gist en meiose
JoVE Journal
Genetics
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JoVE Journal Genetics
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
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Één molecuul fluorescentie In Situ hybridisatie (smFISH) analyse in Budding vegetatieve groei van gist en meiose

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18,732 Views

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09:28 min

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May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

•

09:28 min
May 25, 2018

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7 Views
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1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

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Dit enkel molecuul fluorescentie in situ hybridisatie protocol is geoptimaliseerd voor het kwantificeren van het aantal molecules van RNA in ontluikende gist tijdens de vegetatieve groei en meiose.

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Cite this Article

Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

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