Journal
/
유전학
/
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
JoVE 신문
유전학
This content is Free Access.
JoVE 신문 유전학
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

18,731 Views

•

09:28 min

•

May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

•

09:28 min
May 25, 2018

•

7 Views
, ,
1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

Automatically generated

This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.

Explore More Videos

Single Molecule RNASmFISHBudding YeastVegetative GrowthMeiosisGene ExpressionSubcellular RNA LocalizationFormaldehyde FixationVanadyl Ribonucleoside ComplexesBuffer BZymolyaseDigestion

Read Article

Cite this Article

Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

Download .ris file

Copy

Share Video

.

Copy