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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

DOI:
10.3791/57774-v

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09:28 min

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May 25, 2018

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, ,
1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Chapters

  • 00:05Title
  • 00:40Sample Fixation and Digestion
  • 03:50Hybridization
  • 05:03Washing and Preparing Cells for Imaging
  • 07:03Results: smFISH Detects Individual mRNA Isoforms of NDC80 in Single Cells
  • 08:40Conclusion

Summary

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자동 번역

This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.

Tags

Single Molecule RNASmFISHBudding YeastVegetative GrowthMeiosisGene ExpressionSubcellular RNA LocalizationFormaldehyde FixationVanadyl Ribonucleoside ComplexesBuffer BZymolyaseDigestion

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