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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

18,731 Views

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09:28 min

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May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

•

09:28 min
May 25, 2018

•

7 Views
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1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

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This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.

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Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

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