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Journal / Genetics / Single Molecule Fluorescence In Situ…
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Genetics

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JoVE Journal Genetics
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
 

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Article DOI: 10.3791/57774-v • 09:28 min • May 25th, 2018
May 25th, 2018 •
1, 2, 1
1Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center, University of California, Berkeley

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Summary
Automatic Translation

This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.

Tags

Single Molecule Fluorescence In Situ Hybridization (smFISH) Budding Yeast Vegetative Growth Meiosis RNA Molecules Gene Expression Subcellular RNA Localization Gene Regulation Robust Highly Reproducible Meiotic Culture Formaldehyde Fixation Digestion Master Mix Vanadyl Ribonucleoside Complexes (VRC) Buffer B
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