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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
Journal JoVE
Génétique
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Journal JoVE Génétique
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

18,746 Views

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09:28 min

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May 25, 2018

DOI:

10.3791/57774-v

DOI:
10.3791/57774-v

•

09:28 min
May 25, 2018

•

7 Views
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1Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, 2Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Summary

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This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.

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Single Molecule RNASmFISHBudding YeastVegetative GrowthMeiosisGene ExpressionSubcellular RNA LocalizationFormaldehyde FixationVanadyl Ribonucleoside ComplexesBuffer BZymolyaseDigestion

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Chen, J., McSwiggen, D., Ünal, E. Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. J. Vis. Exp. (135), e57774, doi:10.3791/57774 (2018).

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