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Génération d’insuline exempte d’échafaudage, en trois dimensions exprimant Pancreatoids de souris progéniteurs pancréatique In Vitro
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09:33 min
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June 02, 2018
DOI:
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine
Summary
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Nous présentons ici un protocole pour produire de l’insuline exprimant 3D pancreatoids murin de progéniteurs pancréatique flottant e10.5 dissocié et le mésenchyme associé.
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Cite this Article
Scavuzzo, M. A., Teaw, J., Yang, D., Borowiak, M. Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro. J. Vis. Exp. (136), e57599, doi:10.3791/57599 (2018).
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