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Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
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09:33 min
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June 02, 2018
DOI:
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine
Summary
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Here, we present a protocol to generate insulin expressing 3D murine pancreatoids from free-floating e10.5 dissociated pancreatic progenitors and the associated mesenchyme.
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Scavuzzo, M. A., Teaw, J., Yang, D., Borowiak, M. Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro. J. Vis. Exp. (136), e57599, doi:10.3791/57599 (2018).
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