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Generación de insulina libre de andamios, tridimensional expresar Pancreatoids de progenitores pancreáticos de ratón In Vitro
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09:33 min
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June 02, 2018
DOI:
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine
Summary
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Aquí, presentamos un protocolo para generar insulina expresando 3D pancreatoids murino de e10.5 flotante disociado progenitores pancreático y el mesenchyme asociado.
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Scavuzzo, M. A., Teaw, J., Yang, D., Borowiak, M. Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro. J. Vis. Exp. (136), e57599, doi:10.3791/57599 (2018).
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