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Generation von Gerüst-frei, dreidimensionale Insulin mit dem Ausdruck Pancreatoids vom Maus Pankreas Stammväter In-vitro-
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09:33 min
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June 02, 2018
DOI:
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine
Summary
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Hier präsentieren wir ein Protokoll, um Insulin mit dem Ausdruck 3D murinen Pancreatoids von Pankreas Stammväter frei schwebenden e10.5 getrennt und die damit verbundenen Mesenchym zu generieren.
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Cite this Article
Scavuzzo, M. A., Teaw, J., Yang, D., Borowiak, M. Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro. J. Vis. Exp. (136), e57599, doi:10.3791/57599 (2018).
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