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Generation af stillads-fri, tre-dimensionelle Insulin udtrykker Pancreatoids fra mus i bugspytkirtlen stamfaderen In Vitro
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation af stillads-fri, tre-dimensionelle Insulin udtrykker Pancreatoids fra mus i bugspytkirtlen stamfaderen In Vitro

DOI:
10.3791/57599-v

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09:33 min

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June 02, 2018

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, , ,
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine

Chapters

  • 00:05Title
  • 00:42Preparation of Mouse Embryonic Day 10.5 Pancreatic Progenitors
  • 06:12Culturing Dissociated Progenitors to Form Pancreatoids
  • 06:50Results: Generation of Pancreatoids that Retain Native Mesenchyme and Develop Endocrine Cells
  • 08:37Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her, en protokol for at generere insulin udtrykker 3D murine pancreatoids fra frit svævende e10.5 adskilles i bugspytkirtlen ophav og den tilknyttede udkom.

Tags

PancreatoidsPancreatic ProgenitorsScaffold-freeThree-dimensionalInsulin-expressingMouseEndocrine CellsMesenchymeDissectionE10.5DispaseTrypsinIACUCUterusYolk SacPBS

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