Journal
/
Developmental Biology
/
将毛细血管纳入内皮细胞珠发芽试验
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
Please note that all translations are automatically generated. Click here for the English version.

将毛细血管纳入内皮细胞珠发芽试验

DOI:
10.3791/57309-v

•

06:21 min

•

February 16, 2018

•

, , ,
1Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill, 3Department of Medicine,University of North Carolina at Chapel Hill

Chapters

  • 00:04Title
  • 00:41Endothelial Cell Microcarrier Bead Coating
  • 02:31Gel Implantation
  • 04:57Results: Representative Microcarrier Bead-coating
  • 05:30Conclusion

Summary

Automatic Translation

Automatic Translation

本协议提出了一种新的体外微珠分析方法, 更恰当地模拟体内的过程, 并结合毛细血管。这种修改使珠粒化验更忠实地重述内皮细胞和壁画细胞之间的异型细胞相互作用, 对血管生成至关重要。

Tags

PericytesEndothelial CellsAngiogenesisSprouting AssayHeterotypic Cellular InteractionsMicrocarrier BeadsCell CultureProliferationMigrationAnastomosis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

内皮细胞迁移评估暴露于有毒化学品后，

小鼠胚胎Hemogenic内皮细胞的分离

新型乳腺脂肪移植技术可视化血管内皮干细胞的血管生成

从小鼠骨骼肌分离I型和II型周细胞

单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析

无珠光高音喇叭在食子胚胎中的细胞力学探测

微流控法评价人诱导的多能干细胞内皮细胞 (hipsc-ec) 对白细胞粘附的评价

人类多能干细胞有效分化为肝细胞

用于量化 iPSC 衍生内皮孕激素的血管生成潜力的体外 3D 模型和计算管道

使用由内皮结肠形成细胞和间质干细胞形成的共培养球体的三维血管生成测定系统

Read Article