Rivista
/
Biologia dello sviluppo
/
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

7,814 Views

•

06:21 min

•

February 16, 2018

DOI:

10.3791/57309-v

DOI:
10.3791/57309-v

•

06:21 min
February 16, 2018

•

15 Views
, , ,
1Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill, 3Department of Medicine,University of North Carolina at Chapel Hill

Summary

Automatically generated

This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.

Explore More Videos

PericytesEndothelial CellsAngiogenesisSprouting AssayHeterotypic Cellular InteractionsMicrocarrier BeadsCell CultureProliferationMigrationAnastomosis

Read Article

Cite this Article

Azam, S. H., Smith, M., Somasundaram, V., Pecot, C. V. Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay. J. Vis. Exp. (132), e57309, doi:10.3791/57309 (2018).

Download .ris file

Copy

Share Video

.

Copy