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Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

7,814 Views

06:21 min

February 16, 2018

DOI:

06:21 min
February 16, 2018

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Summary

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This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.

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