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Isolation of Murine Embryonic Hemogenic Endothelial Cells
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Murine Embryonic Hemogenic Endothelial Cells

Isolation of Murine Embryonic Hemogenic Endothelial Cells

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08:56 min

June 17, 2016

DOI:

08:56 min
June 17, 2016

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Summary

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Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.

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