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Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay
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Biologie du développement
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Journal JoVE Biologie du développement
Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay

7,829 Views

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06:21 min

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February 16, 2018

DOI:

10.3791/57309-v

DOI:
10.3791/57309-v

•

06:21 min
February 16, 2018

•

15 Views
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1Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 2Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill, 3Department of Medicine,University of North Carolina at Chapel Hill

Summary

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This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis.

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PericytesEndothelial CellsAngiogenesisSprouting AssayHeterotypic Cellular InteractionsMicrocarrier BeadsCell CultureProliferationMigrationAnastomosis

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Cite this Article

Azam, S. H., Smith, M., Somasundaram, V., Pecot, C. V. Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay. J. Vis. Exp. (132), e57309, doi:10.3791/57309 (2018).

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