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パラフィン包埋と顕微解析の微生物コロニー バイオ フィルムの薄い断面
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Immunology and Infection
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JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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パラフィン包埋と顕微解析の微生物コロニー バイオ フィルムの薄い断面

日本語

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13,835 Views

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08:52 min

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March 23, 2018

DOI:

10.3791/57196-v

DOI:
10.3791/57196-v

•

08:52 min
March 23, 2018

•

7 Views
, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Summary

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固定、パラフィン包埋、および微生物コロニー バイオ フィルムの薄い断面技法について述べる。作製した試料では、顕微鏡によるバイオ フィルムの基礎部分やレポーターの発現パターンを視覚化できます。

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Cite this Article

Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).

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