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Paraffin einbetten und dünn schneiden von mikrobiellen Kolonie Biofilmen für die mikroskopische Analyse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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Paraffin einbetten und dünn schneiden von mikrobiellen Kolonie Biofilmen für die mikroskopische Analyse

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13,835 Views

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08:52 min

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March 23, 2018

DOI:

10.3791/57196-v

DOI:
10.3791/57196-v

•

08:52 min
March 23, 2018

•

7 Views
, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Summary

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Wir beschreiben, Fixierung, Paraffin einbetten und dünne Stationspunkte Techniken für mikrobielle Kolonie Biofilmen. In vorbereiteten Proben können Biofilm Unterkonstruktion und Reporter Expressionsmuster von Mikroskopie visualisiert werden.

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Cite this Article

Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).

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