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Immunology and Infection
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Paraffine insluiten en dunne afdelen van microbiële kolonie Biofilms voor microscopische analyse
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Immunology and Infection
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JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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Paraffine insluiten en dunne afdelen van microbiële kolonie Biofilms voor microscopische analyse

Nederlands

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13,848 Views

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08:52 min

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March 23, 2018

DOI:

10.3791/57196-v

DOI:
10.3791/57196-v

•

08:52 min
March 23, 2018

•

7 Views
, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Summary

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We beschrijven fixatie, paraffine insluiten en dunne vectorafbeeldingsbestanden technieken voor microbiële kolonie biofilms. In bereid monsters, kunnen biofilm substructuur en verslaggever expressiepatronen worden gevisualiseerd door microscopie.

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Cite this Article

Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).

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