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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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March 23, 2018
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We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.
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Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).
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