Journal
/
Immunology and Infection
/
Inclusione in paraffina e sezionamento sottile di biofilm di colonie microbiche per l'analisi al microscopio
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
Please note that all translations are automatically generated. Click here for the English version.

Inclusione in paraffina e sezionamento sottile di biofilm di colonie microbiche per l'analisi al microscopio

italiano

Automatically Generated

13,835 Views

•

08:52 min

•

March 23, 2018

DOI:

10.3791/57196-v

DOI:
10.3791/57196-v

•

08:52 min
March 23, 2018

•

7 Views
, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Summary

Automatically generated

Descriviamo la fissazione, inclusione in paraffina e sottili tecniche di sezionamento per colonie microbiche biofilm. In campioni preparati, modelli di espressione di sottostruttura e reporter di biofilm possono essere visualizzati da microscopia.

Explore More Videos

Paraffin EmbeddingThin SectioningMicrobial Colony BiofilmsMicroscopic AnalysisGene ExpressionFluorescent ProteinBiofilm ArchitectureExopolymeric SubstancesAgar Tryptone SolutionP. AeruginosaColony BiofilmsEmbedding CassetteFixativePBSMicroscopy

Read Article

Cite this Article

Cornell, W. C., Morgan, C. J., Koyama, L., Sakhtah, H., Mansfield, J. H., Dietrich, L. E. Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis. J. Vis. Exp. (133), e57196, doi:10.3791/57196 (2018).

Download .ris file

Copy

Share Video

.

Copy