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Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

DOI:
10.3791/57196-v

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08:52 min

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March 23, 2018

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, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Bölümler

  • 00:04Başlık
  • 00:49Growth of Pseudomonas aeruginosa Colony Biofilms and Overlaying Colonies with Agar
  • 02:33Fixation and Sample Processing
  • 03:02Sample Embedding and Sectioning
  • 05:23Heat Fixing, Rehydration, and Mounting
  • 06:48Results: Microscopic Analysis of Microbial Colony Biofilms
  • 08:01Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We describe fixation, paraffin embedding, and thin sectioning techniques for microbial colony biofilms. In prepared samples, biofilm substructure and reporter expression patterns can be visualized by microscopy.

Etiketler

Paraffin EmbeddingThin SectioningMicrobial Colony BiofilmsMicroscopic AnalysisGene ExpressionFluorescent ProteinBiofilm ArchitectureExopolymeric SubstancesAgar Tryptone SolutionP. AeruginosaColony BiofilmsEmbedding CassetteFixativePBSMicroscopy

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