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Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

9,536 Views

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09:33 min

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March 29, 2018

DOI:

10.3791/57099-v

DOI:
10.3791/57099-v

•

09:33 min
March 29, 2018

•

5 Views
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1The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.

Özet

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This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.

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3D RenderingImmunolabeledClarifiedPlacental Villous Vascular NetworksVascular AnalysisImmunolabelingCD31CK7Fluorescent LabelingMicroscopyPlacental Growth RestrictionPreterm Birth

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Cite this Article

Merz, G., Schwenk, V., Shah, R., Salafia, C., Necaise, P., Joyce, M., Villani, T., Johnson, M., Crider, N. Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks. J. Vis. Exp. (133), e57099, doi:10.3791/57099 (2018).

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