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Chapters
- 00:05Title
- 00:52In Vivo Crosslinking of Proteins to DNA
- 02:19Making Yeast Lysates
- 04:08Immunoprecipitation (IP) of Modified Histones
- 05:11Washing IPs, Eluting Histone-DNA Complexes and Reversing Protein-DNA Crosslinks
- 07:24Quantitative PCR (qPCR) to Detect Enriched Genomic Regions
- 08:46Results: ChIP Reveals Histone Modification Status at ant Genomic Region
- 10:17Conclusion
Tags
Keywords:
Chromatin ImmunoprecipitationChIPHistone ModificationsSaccharomyces CerevisiaeGene Expression ControlEnvironmental ConditionsChromatin Binding ProteinsEpitope TagsAntibodiesYeast CellsYPD MediumOptical DensityFormaldehydeGlycineChIP Lysis BufferPMSFProtease InhibitorGlass BeadsBead-beating
