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酵母からヒストン修飾のクロマチン免疫沈降 (チップ)
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Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
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酵母からヒストン修飾のクロマチン免疫沈降 (チップ)

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12,428 Views

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11:06 min

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December 29, 2017

DOI:

10.3791/57080-v

DOI:
10.3791/57080-v

•

11:06 min
December 29, 2017

•

4 Views
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1Department of Biological Sciences,University of Maryland

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Chromatin ImmunoprecipitationChIPHistone ModificationsSaccharomyces CerevisiaeGene Expression ControlEnvironmental ConditionsChromatin Binding ProteinsEpitope TagsAntibodiesYeast CellsYPD MediumOptical DensityFormaldehydeGlycineChIP Lysis BufferPMSFProtease InhibitorGlass BeadsBead-beating

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Jezek, M., Jacques, A., Jaiswal, D., Green, E. M. Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae. J. Vis. Exp. (130), e57080, doi:10.3791/57080 (2017).

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