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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
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Genética
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JoVE Journal Genética
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

12,469 Views

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11:06 min

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December 29, 2017

DOI:

10.3791/57080-v

DOI:
10.3791/57080-v

•

11:06 min
December 29, 2017

•

12 Views
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1Department of Biological Sciences,University of Maryland

Summary

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Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.

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Chromatin ImmunoprecipitationChIPHistone ModificationsSaccharomyces CerevisiaeGene Expression ControlEnvironmental ConditionsChromatin Binding ProteinsEpitope TagsAntibodiesYeast CellsYPD MediumOptical DensityFormaldehydeGlycineChIP Lysis BufferPMSFProtease InhibitorGlass BeadsBead-beating

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Jezek, M., Jacques, A., Jaiswal, D., Green, E. M. Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae. J. Vis. Exp. (130), e57080, doi:10.3791/57080 (2017).

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