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Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
Chapters
- 00:05Title
- 00:52In Vivo Crosslinking of Proteins to DNA
- 02:19Making Yeast Lysates
- 04:08Immunoprecipitation (IP) of Modified Histones
- 05:11Washing IPs, Eluting Histone-DNA Complexes and Reversing Protein-DNA Crosslinks
- 07:24Quantitative PCR (qPCR) to Detect Enriched Genomic Regions
- 08:46Results: ChIP Reveals Histone Modification Status at ant Genomic Region
- 10:17Conclusion
Here, we describe a protocol for chromatin immunoprecipitation of modified histones from the budding yeast Saccharomyces cerevisiae. Immunoprecipitated DNA is subsequently used for quantitative PCR to interrogate the abundance and localization of histone post-translational modifications throughout the genome.
