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November 21, 2018
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Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.
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Cardamone, M. D., Orofino, J., Labadorf, A., Perissi, V. Chromatin Immunoprecipitation of Murine Brown Adipose Tissue. J. Vis. Exp. (141), e58682, doi:10.3791/58682 (2018).
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