Journal
/
Genetics
/
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

English

Automatically Generated

17,875 Views

•

11:39 min

•

June 17, 2017

DOI:

10.3791/55907-v

DOI:
10.3791/55907-v

•

11:39 min
June 17, 2017

•

8 Views
, ,
1Institute of Biochemistry,University of Giessen

Summary

Automatically generated

This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.

Explore More Videos

Chromatin ImmunoprecipitationChIPMouse T-cell LinesHistone MarksChromatin BiologyChromatin ShearingFormaldehyde CrosslinkingGlycine QuenchingSonicationFocused UltrasonicatorLysis BufferCell PelletSupernatant

Read Article

Cite this Article

Giaimo, B. D., Ferrante, F., Borggrefe, T. Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines. J. Vis. Exp. (124), e55907, doi:10.3791/55907 (2017).

Download .ris file

Copy

Share Video

.

Copy