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Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

DOI:
10.3791/56866-v

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08:50 min

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March 14, 2018

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, , , , , , , ,
1Center for Metabolic and Vascular Biology,Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences,Arizona State University, 3Department of Physics,Arizona State University, 4Center for Biological Physics,Arizona State University, 5Biodesign Institute,Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences,University of Notre Dame, 7Advanced Imaging Center,HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility,European Molecular Biology Laboratory

Chapters

  • 00:05Title
  • 00:47Preparing Acid-cleaned Cover Glass
  • 01:59Preparing of Fusogenic Optical-quality Surfaces
  • 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
  • 03:58Fabricating Glass Bottom Dishes
  • 04:55Collecting Thioglycollate-elicited Macrophages
  • 06:15Results: Macrophage Fusion on Glass Surfaces
  • 07:51Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.

Tags

Optical GlassMacrophage FusionMicroscopyAcid CleaningParaffin CoatingGold TEM Grid

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