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マクロファージの融合を研究するための光学的品質ガラス表面を加工
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
DOI:

08:50 min

March 14, 2018

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Chapters

  • 00:05Title
  • 00:47Preparing Acid-cleaned Cover Glass
  • 01:59Preparing of Fusogenic Optical-quality Surfaces
  • 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
  • 03:58Fabricating Glass Bottom Dishes
  • 04:55Collecting Thioglycollate-elicited Macrophages
  • 06:15Results: Macrophage Fusion on Glass Surfaces
  • 07:51Conclusion

Summary

Automatic Translation

このプロトコルを記述する生きている標本のマクロファージの融合の監視に使用することができ、固定標本の超解像顕微鏡の長鎖炭化水素を含む化合物を吸着した光学的品質のガラス表面の作製.

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