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Fabricação de superfícies de vidro de qualidade óptica para estudar a fusão do macrófago
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1Center for Metabolic and Vascular Biology,Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences,Arizona State University, 3Department of Physics,Arizona State University, 4Center for Biological Physics,Arizona State University, 5Biodesign Institute,Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences,University of Notre Dame, 7Advanced Imaging Center,HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility,European Molecular Biology Laboratory
Chapters
- 00:05Title
- 00:47Preparing Acid-cleaned Cover Glass
- 01:59Preparing of Fusogenic Optical-quality Surfaces
- 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
- 03:58Fabricating Glass Bottom Dishes
- 04:55Collecting Thioglycollate-elicited Macrophages
- 06:15Results: Macrophage Fusion on Glass Surfaces
- 07:51Conclusion
Este protocolo descreve a fabricação de superfícies de vidro ótico-qualidade adsorvido com compostos contendo hidrocarbonetos de cadeia longa que podem ser usados para monitorar fusão macrófago de espécimes vivos e permite a microscopia de super-resolução de espécimes fixos .
