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Fabrication de Surfaces de verre de qualité optique pour étudier la Fusion des macrophages
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1Center for Metabolic and Vascular Biology,Mayo Clinic, 2Molecular and Cellular Biosciences, School of Life Sciences,Arizona State University, 3Department of Physics,Arizona State University, 4Center for Biological Physics,Arizona State University, 5Biodesign Institute,Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences,University of Notre Dame, 7Advanced Imaging Center,HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility,European Molecular Biology Laboratory
Chapters
- 00:05Title
- 00:47Preparing Acid-cleaned Cover Glass
- 01:59Preparing of Fusogenic Optical-quality Surfaces
- 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
- 03:58Fabricating Glass Bottom Dishes
- 04:55Collecting Thioglycollate-elicited Macrophages
- 06:15Results: Macrophage Fusion on Glass Surfaces
- 07:51Conclusion
Ce protocole décrit la fabrication de surfaces de verre de qualité optique adsorbé avec des composés contenant des hydrocarbures à chaîne longue qui peuvent être utilisés pour surveiller la fusion des macrophages de spécimens vivants et permet de microscopie de Super-résolution d’échantillons fixés .
