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Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion

DOI:
10.3791/56866-v

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08:50 min

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March 14, 2018

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, , , , , , , ,
1Center for Metabolic and Vascular Biology,Mayo Klinik, 2Molecular and Cellular Biosciences, School of Life Sciences,Arizona State University, 3Fizik Bölümü,Arizona State University, 4Center for Biological Physics,Arizona State University, 5Biodesign Institute,Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences,Notre Dame Üniversitesi, 7Advanced Imaging Center,HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility,European Molecular Biology Laboratory

Bölümler

  • 00:05Başlık
  • 00:47Preparing Acid-cleaned Cover Glass
  • 01:59Preparing of Fusogenic Optical-quality Surfaces
  • 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
  • 03:58Fabricating Glass Bottom Dishes
  • 04:55Collecting Thioglycollate-elicited Macrophages
  • 06:15Results: Macrophage Fusion on Glass Surfaces
  • 07:51Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.

Etiketler

Optical GlassMacrophage FusionMicroscopyAcid CleaningParaffin CoatingGold TEM Grid

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