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Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
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Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
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08:50 min
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March 14, 2018
DOI:
1Center for Metabolic and Vascular Biology,Mayo Klinik, 2Molecular and Cellular Biosciences, School of Life Sciences,Arizona State University, 3Fizik Bölümü,Arizona State University, 4Center for Biological Physics,Arizona State University, 5Biodesign Institute,Arizona State University, 6Department of Civil and Environmental Engineering and Earth Sciences,Notre Dame Üniversitesi, 7Advanced Imaging Center,HHMI Janelia Research Campus, 8Advanced Light Microscopy Facility,European Molecular Biology Laboratory
Özet
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This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.
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Faust, J. J., Christenson, W., Doudrick, K., Heddleston, J., Chew, T., Lampe, M., Balabiyev, A., Ros, R., Ugarova, T. P. Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion. J. Vis. Exp. (133), e56866, doi:10.3791/56866 (2018).
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